Part:BBa_R0182

T7 RNAP promoter

T7 promoter with an A->T mutation at the -10 position of the consensus sequence

Usage and Biology

This promoter has a single base mutation from the T7 consensus promoter sequence. The mutation is at the -10 base from A to T. The relative strength of this promoter compared to R0085 (the T7 consensus promoter) was measured as 0.3 by Imburgio and coworkers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]